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Fig.3.A showing Immunocytochemistry-immunofluorescence in a Homo sapiens (Human) sample from the publication: Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
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Overexpression of CXCR4 promotes invasion and migration of non-small cell lung cancer via EGFR and MMP-9.

In Oncology Letters on 1 December 2017 by Zuo, J., Wen, M., et al.

The aim of the present study was to verify whether overexpression of CXC receptor 4 (CXCR4) promotes the invasion and migration of non-small cell lung cancer (NSCLC) via epidermal growth factor receptor (EGFR) and matrix metallopeptidase-9 (MMP-9), and to detect the association between CXCR4, EGFR and MMP-9. The effects of overexpression of CXCR4 on lung cancer cell functions were investigated by migration and invasion assays. Western blotting and zymograph assays were used to analyze the protein expression levels of EGFR and the production of MMP-9, respectively. Immunohistochemistry was applied to analyze the expression of EGFR, CXCR4 and MMP-9 in NSCLC. Statistical analyses were used to detect the associations among EGFR, CXCR4 and MMP-9 in NSCLC. Finally, survival analyses were performed. CXCR4 overexpression enhanced cell motility and invasion. CXCR4 also promoted expression of EGFR and elevated MMP-9 production. CXCR4, EGFR and MMP-9 were highly expressed in NSCLC, and were not identified as associated with age and sex (P>0.05). However, they were associated with tumor differentiation and lymph node metastasis (P<0.05). CXCR4, EGFR and CXCR4 expression were positively associated with one another in NSCLC (P<0.05). In addition, patients with positive expression of CXCR4, EGFR or MMP-9 in tumors exhibited significantly shorter overall survival compared with those with negative expression (P<0.05). In conclusion, CXCR4 overexpression enhanced cell motility and invasion via EGFR and MMP-9. CXCR4, EGFR and MMP-9 were identified as highly expressed in NSCLC, and there was positive correlation among them.

  • Homo sapiens (Human)
  • Cancer Research
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Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.

In Nature Communications on 23 March 2015 by Edri, R., Yaffe, Y., et al.

Decoding heterogeneity of pluripotent stem cell (PSC)-derived neural progeny is fundamental for revealing the origin of diverse progenitors, for defining their lineages, and for identifying fate determinants driving transition through distinct potencies. Here we have prospectively isolated consecutively appearing PSC-derived primary progenitors based on their Notch activation state. We first isolate early neuroepithelial cells and show their broad Notch-dependent developmental and proliferative potential. Neuroepithelial cells further yield successive Notch-dependent functional primary progenitors, from early and midneurogenic radial glia and their derived basal progenitors, to gliogenic radial glia and adult-like neural progenitors, together recapitulating hallmarks of neural stem cell (NSC) ontogeny. Gene expression profiling reveals dynamic stage-specific transcriptional patterns that may link development of distinct progenitor identities through Notch activation. Our observations provide a platform for characterization and manipulation of distinct progenitor cell types amenable for developing streamlined neural lineage specification paradigms for modelling development in health and disease.

  • IF
  • ICC-IF
  • Homo sapiens (Human)
  • Stem Cells and Developmental Biology
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Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.

In Nat Commun on 23 March 2015 by Edri, R., Yaffe, Y., et al.

Fig.3.A
Fig.3.A showing Immunocytochemistry-immunofluorescence in a Homo sapiens (Human) sample from the publication: Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
  • ICC-IF
  • Homo sapiens (Human)
Consecutive isolation of Notch active progenitors recapitulates cortical lamination and glial fates.(a) Combined HES5::eGFP reporter expression and immunostainings of cortical layer specific neuronal markers: Early born neurons expressing TBR1, RELN and CTIP2 (top two panels), and late derived ne... more
Consecutive isolation of Notch active progenitors recapitulates cortical lamination and glial fates.(a) Combined HES5::eGFP reporter expression and immunostainings of cortical layer specific neuronal markers: Early born neurons expressing TBR1, RELN and CTIP2 (top two panels), and late derived neurons expressing SATB2, POU3F2 and CUX1 (bottom two panels), are shown for NE, M-RG and L-RG progenitors that were subjected to neuronal differentiation. Insets for RELN/TBR1 and SATB2/POU3F2 show magnified areas within the image. Inset for CTIP/TUJ1 shows same magnification but a different view of neuronal axons. Images of HES5+ derived neurons are shown. Scale bars: 50 μm for images, 25 μm for Insets. Images of HES5− derived neurons and percentages of all cortical subtypes derived from both HES5+ and HES5− cells are presented in Supplementary Fig. 5. (b) Distribution of relative transcript abundance based on qPCR for selected stage-specific marker gene groups for either deep or upper layer neuronal progeny. Contributions of HES5+ and HES5− populations per each respective stage are shown. Marker gene groups for each progenitor stage were created by collapsing the normalized values of TBR1/RELN, CTIP2/FEZF2 and CUX1/CUX2/SATB2 (see Methods for details). Individual qPCR analyses for all genes tested at all stages are shown in Supplementary Fig. 4. (c) Same as in b. Here, cumulative neuronal marker levels based on relative transcript levels are shown (top). Note the decrease in total neuronal progeny shown in the lower panel, as the glial marker GFAP is upregulated in panel e. (d) Distribution of relative transcript abundance based on qPCR for selected stage-specific marker genes for indicated progenitor or neuronal cell markers. Contributions of HES5+ and HES5− populations per each respective stage from either untreated or DAPT treated cells are shown. Expression levels relative to HPRT of all four conditions (colour coded) were summed per each gene and plotted as a single bar. (e) Top: Combined HES5::eGFP reporter expression and immunostaining of the glial marker GFAP following differentiation of the L-RG stage. Scale bar: 50 μm. Bottom: GFAP transcript level for distinct progenitor stages as assessed by qPCR. Values were obtained from three technical replicates. Statistical analysis: mean±s.e.m. less

Collected and cropped from Nat Commun by CiteAb, provided under a CC-BY license

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